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Santa Cruz Biotechnology
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Millipore
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Abcam
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Upstate Biotechnology Inc
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Image Search Results
Journal: Cell Reports
Article Title: Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CL pro substrate degradome
doi: 10.1016/j.celrep.2021.109892
Figure Lengend Snippet:
Article Snippet: The primary antibodies and dilutions used were: mouse monoclonal anti-SARS-CoV-2 nucleocapsid antibody (1:1,000, Invitrogen, MA5-29981, RRID: AB_2785780 ); rabbit anti-SARS-CoV-1 3CL pro antibody (1:2000, Rockland, 200-401-A51, RRID: AB_828457 ); rabbit polyclonal anti-RPAP1 antibody (1:1,000, Proteintech, 15138-1-AP, RRID: AB_2301137 ); mouse monoclonal anti-PTBP1 antibody (1:500, Biolegend, 630101, 3H7, RRID: AB_2171285 ); rabbit polyclonal anti-MAP4K5 antibody (1:1,000, Cusabio, CSB-PA013440DSR2HU, RRID: AB_2892084 ); rabbit polyclonal anti-CREB1 antibody (1:1,000, Abclonal, A11989, RRID: AB_2758916 ); rabbit polyclonal anti-YAP1 antibody (1:1,000, Abclonal, A11430, RRID: AB_2758556 ); rabbit polyclonal anti-FYCO1 antibody (1:1,000, Cusabio, CSB-PA866262LA01HU, RRID: AB_2892085 ); rabbit polyclonal anti-FAF1 antibody (1:1,000, Abclonal, A2921, RRID: AB_2764739 ); goat polyclonal anti-Gal8 antibody (1:400, R&D Systems, AF1305, RRID: AB_2137229 ); rabbit polyclonal anti-KPNA3 (IMA4) antibody (1:1,000, Abclonal, A8347, RRID: AB_2770124 ); rabbit polyclonal anti-NUP107 antibody (1:1,000, Abclonal, A13110, RRID: AB_2759959 );
Techniques: Virus, Recombinant, Sequencing, Fluorescence, Modification, Protease Inhibitor, Polymer, Staining, Blocking Assay, Binding Assay, Activity Assay, Western Blot, Synthesized, Software, Control, Targeted Proteomics, Microscopy, Spectrophotometry, Mass Spectrometry
Journal: Emerging Microbes & Infections
Article Title: SARS-CoV-2 in the pancreas and the impaired islet function in COVID-19 patients
doi: 10.1080/22221751.2022.2059400
Figure Lengend Snippet: The expression of islet function-related molecules was altered in patients with COVID-19. Immunochemical staining using antibodies against (A) CD36, (B) GLUT2, (C) IRS1, (D) IRS2, (E) PDX1 and (F) PPARG. In the patient with COVID-19 but without SARS-CoV-2 virus in the pancreas (SARS-CoV-2 negative), CD36, GLUT2, IRS2 and PDX1 were widely distributed in the pancreatic serous acinus, the duct (exocrine gland) and the islet (endocrine gland); PPARG was mainly in the islet; and IRS1 was barely detected. In the patient with COVID-19 accompanied by SARS-CoV-2 virus in the pancreas (SARS-CoV-2 positive), CD36, GLUT2, IRS1, IRS2, PDX1 and PPARG were mainly detected in the islets. CD36 was also present in the small vasculature of pancreatic interstitial tissue.
Article Snippet: Following antigen retrieval in EDTA (pH: 9.0), the sections were incubated overnight at 4 °C with primary antibodies against SARS-CoV-2 spike (1:200, GTX632604, Genetex), ACE2 (1:200, GB11267, Servicebio), CD147 (1:200, GB11390-1, Servicebio), PDX1 (1:200, 20989-1-ap, Proteintech), PPAR (1:200, BS-4590R, BIOSS), CD36 (1:200, 18836-1-ap, Proteintech), GLUT2 (1:200, bs-051r, BIOSS), IRS1 (1:200, AF6273, Affinity), or
Techniques: Expressing, Staining
Journal: Cell Metabolism
Article Title: Exome Sequencing Identifies Genes and Gene Sets Contributing to Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC Transcription
doi: 10.1016/j.cmet.2020.05.007
Figure Lengend Snippet:
Article Snippet: Samples were span at 800rpm for 30 s and incubated overnight with 5μl of normal Rabbit IgG (Cell Signaling, 2729) or
Techniques: Virus, Recombinant, Reporter Gene Assay, Plasmid Preparation, Software, CRISPR, Variant Assay
Journal: eLife
Article Title: IRS-1 acts as an endocytic regulator of IGF-I receptor to facilitate sustained IGF signaling
doi: 10.7554/eLife.32893
Figure Lengend Snippet:
Article Snippet: Antibody ,
Techniques: Transduction, Recombinant, Plasmid Preparation, Sequencing, Control, shRNA, Protease Inhibitor, Software